Tissue collection

Formalin fixed paraffin embedded (FPPE)

FFPE tissue samples are fixed in 10% neutral-buffered formalin within 30 minutes of surgical excision. Tissue samples are fixed for 18-20 hours and embedded in IHC-grade paraffin. Standard sample size is 1 x 1 x 0.5 cm. The fixation agent and the embedding media can be customized upon request.

The following standard clinical information will be provided for each sample: 

  • Basic information (age, ethnicity, height, weight, gender) 
  • Date of sample procurement 
  • Clinical diagnosis (tumor location, type, stage, grade, TMN) 

The pathology report can include: biomarker status (ER, PR, Her2), the location of distant metastasis, the number of analyzed lymph nodes, the number of positive (metastatic) lymph nodes, tumor size.

Fresh Frozen (FF)

Fresh frozen tissue samples are snap-frozen in liquid nitrogen within 30 minutes of from devascularization. The standard sample weight is 200-300 mg. Freshly frozen tissue sample collection is performed strictly following the BiCell Analytics SOP. Samples can be collected in embedding media upon request.

The following standard clinical information will be provided for each sample: 

  • Basic information (age, ethnicity, height, weight, gender) 
  • Date of sample procurement 
  • Clinical diagnosis (tumor location, type, stage, grade, TMN) 
  • Pathology report  

Quality control procedure

The initial review of the samples is performed by the Quality Control Manager at the Pathology laboratory. 

The initial review assesses and confirms the following:

  • Proper sample format and condition
  • Sample volume or size (weight)
  • Proper labeling

Pathology review of the samples 

Primary quality control practice includes a morphologic analysis of H&E stained slides;

An independent MD pathologist performs the review.

Pathology report (made by a certified MD Pathologist) includes:

  • Tissue type and assessment of diagnosis;
  • Tumor type;
  • Presence of tumor;
  • Cell content of tumor and stromal area by percentage;
  • Percentage of tumor necrosis;
  • The presence of artifacts.